| Primary Identifier | MGI:7525660 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Syt7 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using homologous recombination, exon 2 of the mouse Syt7 gene that encodes the transmembrane region was modified to introduce a haemagglutinin (HA) tag between the Syt7 protein N-terminal and the transmembrane region; in addition, a loxP site was introduced into the 5â² intron and a neomycin resistance cassette (NEO) that was flanked by frt1 sites and was followed by a second loxP site introduced in the 3â² intron. Flp recombinase removed the frt flanked neo-selection cassette. Cre recombinase removed the loxP-flanked exon 2. |
