| Primary Identifier | MGI:7522248 | Allele Type | Endonuclease-mediated |
| Attribute String | Reporter | Gene | Foxi3 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing used guide RNAs [CCC CCGGGATGGCTCTGATATA] to insert an GSG-P2A peptide sequence and mVenus fluorescent protein coding sequence into the 3' UTR. Foxi3 transcript Foxi3-201 (ENSMUST00000069634.6) was used as reference for the exon number and guide sequences. Donor DNA contained two nucleotide changes (C to T and C to G) to prevent the inserted sequence from being targeted by the Foxi3 gRNA. |
