| Primary Identifier | MGI:7516132 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence | Gene | Foxo1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site, a Foxo1 cDNA, a roxP site, a second loxP site, and poly(A) signal sequence, were inserted into the 5' UTR of a mutated exon 1. The mutation involved changing threonine codon 24 (ACC) to alanine (p.T24A). This allele expresses the wild-type transcript, but after Cre-mediated deletion of the cDNA sequence it expresses the mutated transcript. The mutation changes a phosphorylatable residue in the encoded peptide to a phosphoblocker, which blocks the inactivation of the protein. |
