Primary Identifier | MGI:7532845 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Lhx4 |
Transmission | Germline | Strain of Origin | C57BL/6J x 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector was constructed containing a floxed exon 3 of the Lhx4 gene, an FRT-flanked En2 splicing acceptor-IRES-tdTomatopGK-Neo (tdT-Neo) cassette into the intron upstream of the exon 3 of the Lhx4. FRT-flanked En2 splicing acceptor-IRES-tdTomatopGK-Neo (tdT-Neo) cassette was removed by FLPe recombinase leaving loxP flanked exon 3. The loxP flanked exon 3 was then removed by cre recombinase removing Exon 3 and resulting in a frameshift. This is a null allele. |