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Allele : Gt(ROSA)26Sor<em1(CAG-PACS1*R203W)Gath> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Gary Thomas
Primary Identifier  MGI:7518813 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  CRISPR/cas9 endonuclease-mediated genome editing is used to insert hemagluttin-tagged human PACS1 cDNA with the R203W (arginine to tryptophan mutation), followed by an IRES and a GFP reporter under the control of the widespread CAG promoter. A loxP-flanked neomycin STOP precedes the PACS1 sequence. The GFP is too low to be detected and was only used for PCR genotyping.
  • mutations:
  • Nucleotide substitutions,
  • Insertion
  • synonyms:
  • R26<P1R203W,
  • R26<P1R203W,
  • PACS1<R203W>,
  • ROSA26-LSL-PACS1-R203W,
  • ROSA26-LSL-PACS1-R203W,
  • PACS1<R203W>
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0 Mutation Involves

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