| First Author | Gu M | Year | 2022 |
| Journal | Int J Mol Sci | Volume | 23 |
| Issue | 22 | PubMed ID | 36430183 |
| Mgi Jnum | J:338940 | Mgi Id | MGI:7410056 |
| Doi | 10.3390/ijms232213703 | Citation | Gu M, et al. (2022) Myostatin Knockout Affects Mitochondrial Function by Inhibiting the AMPK/SIRT1/PGC1alpha Pathway in Skeletal Muscle. Int J Mol Sci 23(22) |
| abstractText | Myostatin (Mstn) is a major negative regulator of skeletal muscle mass and initiates multiple metabolic changes. The deletion of the Mstn gene in mice leads to reduced mitochondrial functions. However, the underlying regulatory mechanisms remain unclear. In this study, we used CRISPR/Cas9 to generate myostatin-knockout (Mstn-KO) mice via pronuclear microinjection. Mstn-KO mice exhibited significantly larger skeletal muscles. Meanwhile, Mstn knockout regulated the organ weights of mice. Moreover, we found that Mstn knockout reduced the basal metabolic rate, muscle adenosine triphosphate (ATP) synthesis, activities of mitochondrial respiration chain complexes, tricarboxylic acid cycle (TCA) cycle, and thermogenesis. Mechanistically, expressions of silent information regulator 1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK) were down-regulated, while peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) acetylation modification increased in the Mstn-KO mice. Skeletal muscle cells from Mstn-KO and WT were treated with AMPK activator 5-aminoimidazole-4-carboxamide riboside (AICAR), and the AMPK inhibitor Compound C, respectively. Compared with the wild-type (WT) group, Compound C treatment further down-regulated the expression or activity of pAMPK, SIRT1, citrate synthase (CS), isocitrate dehydrogenase (ICDHm), and alpha-ketoglutarate acid dehydrogenase (alpha-KGDH) in Mstn-KO mice, while Mstn knockout inhibited the AICAR activation effect. Therefore, Mstn knockout affects mitochondrial function by inhibiting the AMPK/SIRT1/PGC1alpha signaling pathway. The present study reveals a new mechanism for Mstn knockout in regulating energy homeostasis. |
