Primary Identifier | MGI:7545057 | Allele Type | Targeted |
Attribute String | Conditional ready, Epitope tag, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | B6(Cg)-Tyr<c-2J> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct containing from 5' to 3': a CAG promoter, a loxP-STOP-loxP, a tandem mitochondrial targeting sequences (MTS) consisting of the Lactobacillus brevis COXIV and the Homo sapiens ACO2 MTS as well as a FLAG tag epitope, and the Streptococcus thermophilus-derived bifunctional glutathione synthetase (mito-GshF) cDNA followed by a stop codon (TAA) was inserted into the Gt(ROSA)26Sor locus. There also is a neomycin cassette in the construct. |