| Primary Identifier | MGI:7620226 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Neurog3 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into intron 1 and an FRT site flanked puromycin resistance gene cassette, a second loxP site, and a copy of exon 2 (with CHYSEL (cis-acting hydrolase element) sequence and the EYFP gene inserted in-frame at the 3' end of the CDS and mutations changing six serine codons to alanine) were inserted downstream of the gene. The puro cassette was removed through subsequent Flp-mediated recombination. The floxed endogenous exon 2 was deleted through after Cre-mediated recombination, allowing expression of the reporter peptide in conjunction with the mutated (unphosphorylatable) neurogenin 3 peptide from the exon 2 copy. |
