Primary Identifier | MGI:7565731 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag | Gene | Prdm14 |
Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
Is Wild Type | false |
molecularNote | This allele was generated at The Centre for Phenogenomics by injecting Cas9 protein and a synthetic crRNA with sequence CCGCAAAACUCAGCUUGCGA targeting the 5' side of exon ENSMUSE00000316434 and a single-strand oligonucleotide encoding a 3xHis tag. The 3xHis tag was inserted adjacent to the ATG codon of Prdm14 (c.3_4insCACCACCACCACCACCAC). |