| Primary Identifier | MGI:7560638 | Allele Type | Endonuclease-mediated |
| Gene | Birc3 | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was generated at the same time as Birc2em1Haka. |
| molecularNote | CRISPR/Cas9 technology generated an 8-bp deletion leading to a frameshift that prolongs the mutant protein by 23 amino acids changing the phenylalanine at position 600 to a leucine (G601QfsTer34). This deletion was generated by simultaneously injecting C57BL/6N zygotes with two sgRNAs targeting Birc2 (5â-TCTGCAGGGGGACAATCAAG-3â) and Birc3 (5â-ATCTGTAGAGGGACCATCAA-3â) and was generated at the same time as the Birc2em1Haka allele. |
