| Primary Identifier | MGI:7572835 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Snca |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector consisting of loxP-flanked human alpha-synuclein, followed by a C-terminally truncated human alpha-synuclein lacking 21 amino acids, IRES, a placental alkaline phosphatase (pLAP)reporter gene, and an FRT-flanked neomycin gene was targeted to exon 3. The neomycin selection cassette was removed via flp-mediated recombination. Cre-mediated recombination removed the human alpha-synuclein resulting in the expression of the C-terminally truncated protein. RT-PCR confirmed the expression of the expected 503 bp human mutant transcript. The truncation terminates with aspartic acid at residue 119 (120 CCT to TAA) and favors fibrillary aggregate formation. |
