| Primary Identifier | MGI:7607009 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Fcgr2b |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using a CRISPR/Cas9 approach, an âactive genetic cassetteâ (AGC) was used to remove the endogenous mouse gene coding region and replace it with a functional human FCGR2B coding region under the control of the endogenous mouse promoter. The AGC included a Kozak consensus translational initiation sequence and human FCGR2B cDNA mini-gene cassette (HsFCGR2B), 1101 bp native human FCGR2B3âUTR (HsFCGR2B 3âUTR) U6 promoter, gRNA, Stop-FRT cassette, and 3âHR. |
