| Primary Identifier | MGI:7576709 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Modified regulatory region | Gene | Rr467 |
| Strain of Origin | (C57BL/6J x SJL/J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology replaced one of the cis-regulatory elements marked as DNase I-hypersensitive sites around the mouse Cbln2 locus designated as the Cbln2 enhancer E2 (1,005 bp) with the corresponding human CBLN2 enhancer E2 (chr18: 72,530,473â72,531,324, GRCh38/hg38). Quantitative RT-PCR showed increased RNA expression of mouse Cbln2 in neonatal neocortex in homozygous mice. |
