| Primary Identifier | MGI:7620430 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Srsf2 |
| Transmission | Germline | Strain of Origin | C57BL/6 x 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP and a VloxP site were inserted into the 5' UTR, a VloxP site, a lox2272 site, a copy of the lox site flanked portion of exon 1, in reverse orientation, where proline codon 95 (CCG) was changed to histidine (CAC) (c.284_285delCGinsAT:p.P95H), and an FRT site flanked neomycin resistance gene cassette, were inserted into intron 1. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses wildtype peptides and only after Cre-mediated deletion of the endogenous exon 1 and flipping of the mutated duplicate, will it express the mutated peptide. |
