| Primary Identifier | MGI:7581011 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Dnase1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology using sgRNA 5â-TGACATCGCTGTTATCCAAG-3â generated a 65 bp deletion from intron 2 into exon 3. This deletion does not allow splicing into exon 3, and potential alternative splicing into exons 4, 5 or 6 containing the active sites for Dnase1 enzymatic activity lead to frameshift mutations and premature stop codons. |
