| Primary Identifier | MGI:7595847 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Nudt15 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Arginine codon 138 (CGC) in exon 5 was changed to cysteine (TGC) (c.412C>T, p.R138C) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of the human p.R139C mutation (c.415C>T, SNP rs116855232) associated with severe thiopurine-induced leukocytopenia. Transcript and protein expression from this allele is the same as wild-type. |
