| First Author | Liu JY | Year | 2019 |
| Journal | Proc Natl Acad Sci U S A | Volume | 116 |
| Issue | 7 | Pages | 2603-2611 |
| PubMed ID | 30683717 | Mgi Jnum | J:272509 |
| Mgi Id | MGI:6280561 | Doi | 10.1073/pnas.1818313116 |
| Citation | Liu JY, et al. (2019) Cells exhibiting strong p16 (INK4a) promoter activation in vivo display features of senescence. Proc Natl Acad Sci U S A 116(7):2603-2611 |
| abstractText | The activation of cellular senescence throughout the lifespan promotes tumor suppression, whereas the persistence of senescent cells contributes to aspects of aging. This theory has been limited, however, by an inability to identify and isolate individual senescent cells within an intact organism. Toward that end, we generated a murine reporter strain by "knocking-in" a fluorochrome, tandem-dimer Tomato (tdTom), into exon 1alpha of the p16 (INK4a) locus. We used this allele (p16 (tdTom) ) for the enumeration, isolation, and characterization of individual p16 (INK4a) -expressing cells (tdTom(+)). The half-life of the knocked-in transcript was shorter than that of the endogenous p16 (INK4a) mRNA, and therefore reporter expression better correlated with p16 (INK4a) promoter activation than p16 (INK4a) transcript abundance. The frequency of tdTom(+) cells increased with serial passage in cultured murine embryo fibroblasts from p16 (tdTom/+) mice. In adult mice, tdTom(+) cells could be readily detected at low frequency in many tissues, and the frequency of these cells increased with aging. Using an in vivo model of peritoneal inflammation, we compared the phenotype of cells with or without activation of p16 (INK4a) and found that tdTom(+) macrophages exhibited some features of senescence, including reduced proliferation, senescence-associated beta-galactosidase (SA-beta-gal) activation, and increased mRNA expression of a subset of transcripts encoding factors involved in SA-secretory phenotype (SASP). These results indicate that cells harboring activation of the p16 (INK4a) promoter accumulate with aging and inflammation in vivo, and display characteristics of senescence. |
