| Primary Identifier | MGI:7616486 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Cdc27 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Serine codon 93 (TCT) in exon 4 was changed to phenylalanine (TTT) (p.S93F). A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and second loxP site were inserted into intron 5. The neo cassette was removed through subsequent Flp-mediated recombination, leaving exons 4-5 floxed. Mutations in the human ortholog are associated with non-syndromic intellectual disability. |
