| Primary Identifier | MGI:7645676 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Gnao1 |
| Strain of Origin | (C57BL/6 x CBA/Lac)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Glycine codon 203 (GGG) in exon 6 was changed to GGA (c.609G>A) using an sgRNA (targeting GCTTTCCCTGACTCCCTGC) and an ssODN template with CRISPR/Cas9 technology. The mutation makes the codon identical to the human G203 codon, a variant in which (SNP rs587777057) is associated with epileptic seizures and movement disorder. Transcript and protein expression are not affected by the mutation. |
