Primary Identifier | MGI:7645890 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Aspscr1 |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Targeting vector from IKMC Project CSD30881 composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5. The critical exon 5 is thus flanked by loxP sites. The lacZ sequence and selection cassette were removed via flp-mediated recombination, leaving exon 5 floxed. |