| First Author | Chang B | Year | 2024 |
| Journal | MGI Direct Data Submission | Mgi Jnum | J:354548 |
| Mgi Id | MGI:7735167 | Citation | Chang B (2024) The retinal vascularization 9 (rnv9) model. MGI Direct Data Submission |
| abstractText | The retinal neovascularization 9 mutation (rnv9) was identified in an F2 intercross population from the semidominant CWXS/Agl-Gja8<R205G>/Boc (JAX Stock No. 030091) and C57BL/6J (JAX Stock No. 000664). The rnv9 mutant was wildtype for Gja8 and when backcrossed to CWXS/Agl-Gja8<R205G>/Boc the rnv9 phenotype was evident at 3 weeks of age, before the Gja8<R205G> heterozygous cataract phenotype interfered. We mated an rnv9 phenotypic mutant to C57BL/6J and all F1 mice had normal retinas. The rnv9 phenotype was recovered in the F2 generation with multifocal depigmented retinal lesions at about 3 weeks of age. We developed a C57BL/6J (B6) congenic strain, B6.Cg-rnv9/Boc (JAX Stock No. 008808), by backcross-intercross breeding the rnv9 allele onto C57BL/6J for 5 backcross generations then sibling mating to homozygosity. Mice homozygous for the rnv9 showed a very good retinal neovascularization phenotype (Figure 1A) at 4 weeks of age compared with the C57BL/6J control (Figure 1B). The neovascularization process in this model is similar to a type seen in patients with macular degeneration. |
