Primary Identifier | MGI:7705091 | Allele Type | Endonuclease-mediated |
Attribute String | Modified regulatory region | Gene | Rr499 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The Ptf1a 5' enhancer was targeted by two sgRNAs (equivalent to CACAAGTGGCGACATTCCCA and CCGCAGAGCACGCCAGTCCG) using CRISPR/Cas9 technology, resulting in an ~2.4 kb deletion containing the near and far autoregulatory PTF1-binding motifs. This allele was created in conjunction with the Rr500em1Jejo allele. |