| Primary Identifier | MGI:7715238 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The allele has the mouse immunoglobulin heavy chain complex (Igh) region variable (V), diversity (D) and joining (J) segments deleted and replaced with a human IGH V-D-J sequence. Specifically, CRISPR/Cas9 genome editing in 129S6/SvEvTac-derived CMTI-1 embryonic stem (ES) cells was used in two separate steps to deliver into the mouse Igh locus a ~139 kb sequence containing an array of 41 IGH V segments plus a fully functional complement of 27 IGH D and 6 IGH J gene segments. |
