Primary Identifier | MGI:7712919 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Del(5Gbp4-Gbp8)1Mansm |
Strain of Origin | Not Specified | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology using sgRNA 5â-GGTGGAGGCGGGGTATGGTGAGG-3â and 5â-CATGGAGAGTGGAATTTGAGAGG-3â targeting upstream of the Gbp4 gene and sgRNAs 5â-AGCTCACTGCTTCTCCATACTGG-3â and 5â-TTCATCTCTTGTAAGATGGGAGG-3â targeting downstream of the Gbp8 gene generated a 127 kb deletion from the intergenic region upstream of Gbp4 to the intergenic region downstream of Gbp8 and deleted Gbp4, Gbp9, and Gbp8. |