| Primary Identifier | MGI:7861927 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Cd1d1 |
| Inheritance Mode | Not Specified | Strain of Origin | BALB/cAnNRj |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR-targeting of exon 1 inserted a T in position 17, starting ATG, which induced a downstream STOP codon producing a truncated 19-aa protein. CRISPR technology details: Cas9 mRNA and sgRNAs targeting exon1 were mixed and electroporated (NEPA21) into the pronuclei of the one-cell embryos. SgRNA1 5â²- CCCACAGCAACAGCCATGGT-3â² and SgRNA3 5â²-CCTACCATGGCTG TTGCTGT-3â² |
