Primary Identifier | MGI:7666006 | Allele Type | Endonuclease-mediated |
Attribute String | Reporter | Gene | Cd74 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Using CRISPR/Cas9 technology, a P2A self-cleaving peptide sequence, a red fluorescent protein (tdTomato) sequence, and woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) followed by a polyadenylation signal were inserted to replace the stop codon of the endogenous CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) (Cd74) gene. |