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Allele : Gt(ROSA)26Sor<em4(CAG-Tbx2,-tdTomato)Zyliu> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 4, Zhiyong Liu
Primary Identifier  MGI:7703100 Attribute String  Conditional ready, Epitope tag, Inserted expressed sequence, Reporter
Gene  Gt(ROSA)26Sor Strain of Origin  C57BL/6J
Is Recombinase  false Is Wild Type  false
molecularNote  Using CRISPR/Cas9 technology, a cassette encoding a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG) followed by (from 5' to 3') a floxed STOP cassette and a 3xHA epitope C-terminal tagged T-box 2 (Tbx2) cDNA, a viral 2A oligopeptide (P2A) self-cleaving peptide, followed by a tdTomato sequence and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) fused to a polyadenylation signal were inserted into the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26-CAG-Loxp-stop-Loxp-Tbx2*3 × HA-P2A-tdTomato,
  • Rosa26<Tbx2>,
  • Rosa26-CAG-Loxp-stop-Loxp-Tbx2*3 × HA-P2A-tdTomato,
  • Rosa26<Tbx2>
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0 Mutation Involves

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