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Allele : Gt(ROSA)26Sor<em5(CAG-Tbx2,-Ikzf2)Zyliu> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 4, Zhiyong Liu
Primary Identifier  MGI:7703104 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Epitope tag, Inserted expressed sequence, Reporter Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  Using CRISPR/Cas9 technology, a cassette encoding a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG) followed by (from 5' to 3') a floxed STOP cassette and a 3xHA epitope C-terminal tagged T-box 2 (Tbx2) cDNA, a viral 2A oligopeptide (P2A) self-cleaving peptide, followed by a 3xV5 epitope C-terminal tagged IKAROS family zinc finger 2 (Ikzf2) cDNA, a viral 2A oligopeptide (T2A) self-cleaving peptidee and a EGFP sequence and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) fused to a polyadenylation signal were inserted into the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26-LSL-Tbx2-Ikzf2,
  • Rosa26-LSL-Tbx2-Ikzf2,
  • Rosa26<Tbx2-Ikzf2>,
  • Rosa26<Tbx2-Ikzf2>
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1 Feature

Trail: Allele

Genome

2 Expresses

Trail: Allele

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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