Primary Identifier | MGI:7703105 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag, Inserted expressed sequence | Gene | Lypd1 |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Using CRISPR/Cas9 technology, a 3xV5 tag, a P2A self-cleaving peptide, and the human diphtheria toxin receptor (DTR) sequence immediately upstream of the endogenous stop codon of the Ly6/Plaur domain containing 1 (Lypd1) gene. Of note, besides being correctly targeted into the Lypd1 locus as designed, the targeting vector was randomly inserted in one unknown location in the mouse genome. |