Primary Identifier | MGI:7666524 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | ES cells containing the Col1a1tm13(neo/hygro*)Jae allele were targeted, using Flp recombinase, with a vector to replace the FRT site flanked neomycin resistance gene cassette in the homing cassette downstream of Col1a1 with an FRT site flanked cassette containing Pitx1 distal or pituitary gland enhancer PDE or Pit sequence (GRCm39:chr13:56108910-56111397) followed by the beta-galactosidase reporter gene lacZ, PGK promoter sequence and ATG start site. |