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Allele : Gt(ROSA)26Sor<em2(CAG-LOC4337827*)Mbu> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Meinrad Busslinger
Primary Identifier  MGI:7703958 Allele Type  Endonuclease-mediated
Attribute String  Inserted expressed sequence Gene  Gt(ROSA)26Sor
Strain of Origin  129P2/OlaHsd Is Recombinase  false
Is Wild Type  false
molecularNote  The CAG promoter drives a codon-optimized Oryza sativa F-box transport inhibitor response 1 (TIR1) sequence, linked to an N-terminal nuclear localization signal (NLS), an internal ribosome entry site (IRES) sequence and a puromycin cassette, inserted into intron 1 of the Gt(ROSA)26Sor locus. The allele was generated by introducing the F74G mutation into an existing Rosa26(Tir1) allele CRISPR-Cas9 methodology introduced the F74G mutation into an existing F-box transport inhibitor response 1 (TIR1) protein carried by the Gt(ROSA)26Sortm2(CAG-LOC4337827)Afst allele.
  • mutations:
  • Insertion,
  • Nucleotide substitutions
  • synonyms:
  • Rosa26<Tir1-F74G>,
  • Rosa26<Tir1-F74G>
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Trail: Allele

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0 Mutation Involves

Phenotype

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Trail: Allele




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