| Primary Identifier | MGI:7666156 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Camk2b |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Sequence (100 bp) containing intronic splicing regulation sequence (in 3' end of ENSMUST00000002817 intron 13, ENSMUST00000019133 intron 14, or "global" intron 15 when taking all possible coding exons into account) was removed and replaced with the human equivalent using an sgRNA and a human minigene template with CRISPR/Cas9 technology. The sequence contains species-specific cis-regulatory sequences that determine the branch point strength in the pre-mRNA, which determines whether or not the downstream exon is spliced-in or skipped. |
