| First Author | Chang B | Year | 2024 |
| Journal | MGI Direct Data Submission | Mgi Jnum | J:351807 |
| Mgi Id | MGI:7703891 | Citation | Chang B (2024) The Grm6 |
| abstractText | The spontaneous mutation nob3-2J was discovered in our ocular phenotypes screening program in June 2002 in strain B10.Cg-Hc<1> H2<d> H2-T18<c> Tg(DO11.10)10Dlo/J (JAX Stock No. 003147) that presented with near-normal a-wave but absent both scotopic and photopic b-waves in electroretinogram (ERG) assessment. We mated the mutant mice with C57BL/6J and all F1 mice had normal ERG b-wave. The no ERG b-wave mice were recovered n the F2 generation. The ERG phenotype in this strain is very similar compared to B6.B10(D2)-Grm6nob3/BocJ (JAX Stock No. 016883) which was discovered in B10.Cg-Tg(Igh2k3-83)1Nemz/J (JAX Stock No. 003138). Since this new no ERG b-wave and nob3 were both discovered in B10 related genetic backgrounds, before setting up the mapping crosses, we did a complementation test between this new mutation and nob3 homozygotes and they failed to complement proving them allelic. We named the new no b-wave mutation Grm6nob3-2J and we developed a C57BL/6J (B6) congenic strain, B6.Cg-Grm6nob3-2J/Boc (JAX Stock No. 035326), by backcrossing the Grm6nob3-2J allele onto C57BL/6J for 5 generations then sibling mating to homozygosity. |
