Primary Identifier | MGI:7788441 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6NCrl |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The following elements were inserted into an intron: the CAG promoter, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK and 3x SV40 p(A) signal sequences), followed by cDNA for the protease component (222 AA) of the tobacco etch virus polyprotein TEVgp1, an FRT site, IRES sequence, the EGFP reporter gene, a second FRT site, and bovine GH poly(A) signal sequence. This allele only expresses TEVgp1 and EGFP after Cre-mediated removal of the stop cassette. |