Primary Identifier | MGI:7751124 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6NTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector consists of (from 5' to 3') an FRT3 site, PGK neo polyA cassette, a CAG promoter, a Kozak sequence (GCCACC), the mCherry-GFP-Map1lc3b (mCherry-GFP-LC3) coding sequence, a human growth hormone polyA sequence (hGHpA), an additional polyA sequence and a FRT site. Recombination mediated cassette exchange (RMCE) is used to insert the CAG promoter and the open reading frame for the mCherry-GFP-Map1lc3b fusion protein into the Gt(ROSA)26Sor locus. |