| Primary Identifier | MGI:7785121 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Rrm1 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 8-15 were replaced with a construct containing a TA to GC mutation in exon 9, resulting in tyrosine to alanine substitution at amino acid 285 (p.Y285A). In addition, a neomycin cassette consisting of FRT-PGK-gb2-Neo-loxP-FRT, was inserted in the intron between exons 8 and 9. The neomycin selection cassette was removed via flp-mediated recombination. The Y285 residue has been identified as mutated in human cancer samples, is the region encoding the allosteric specificity site, and reduces ribonucleotide reductase activity. |
