| Primary Identifier | MGI:7857092 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus. The Rosa26 promoter in this mouseà was altered by replacing DNA sequences from 228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box. This mouse will facilitate studies of retinoic acid signaling in an intact animal. In this experiment native Rosa26 gene sequence from -228 to +81 will be replaced by a retinoic acid response element (DR5) fused to a TATACerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested. |
