| Primary Identifier | MGI:8235547 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Ctnnd2 |
| Strain of Origin | C57BL/6JCya | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The TAA stop codon was replaced with the P2A-tdTomato-IRES-loxP-6xSV40 pA loxP-DTR cassette. Upstream of the DTR (diphtheria toxin receptor) gene lies a loxP-Stop-loxP cassette that blocks DTR transcription and expression. In the absence of Cre recombinase, the Ctnnd2 promoter drives tdTomato expression, labeling Ctnnd2-positive cells with red fluorescence. Upon Cre recombination, Cre-mediated deletion of the loxP-flanked stop (LSL) element enables DTR expression in Cre-positive cells. Intravenous administration of diphtheria toxin (DT) then selectively ablates these cells. |
