| First Author | Longas MO | Year | 1981 |
| Journal | Biochem J | Volume | 197 |
| Issue | 2 | Pages | 275-82 |
| PubMed ID | 6459778 | Mgi Jnum | J:323612 |
| Mgi Id | MGI:7264271 | Doi | 10.1042/bj1970275 |
| Citation | Longas MO, et al. (1981) Sequential hydrolysis of hyaluronate by beta-glucuronidase and beta-N-acetylhexosaminidase. Biochem J 197(2):275-82 |
| abstractText | 1. Hyaluronate extracted from rooster comb was digested by a mixture of beta-N-acetylhexosaminidase and beta-glucuronidase with simultaneous dialysis for 96 h. 2. The produjct, yielding 99.6% of a mixture of mono- and oligo-saccharides, was purified by gel chromatography and analysed for glucuronic acid, N-acetylglucosamine and other sugars. 3. The oligosaccharide portion was chromatographed on DEAE-cellulose, and the effluent fractions were analysed for glucuronic acid and N-acetylglucosamine, reduced with NaBH4, digested by beta-N-acetylhexosaminidase and subjected to acid hydrolysis and glucosamine determination. 4. GlcNAc-GlcA-GlcNAc, GlcA-GlcA-GlcNAc and GlcA-GlcA-GlcA-GlcNAc were the oligosaccharides obtained, which resulted from the transferase activity of the enzymes and represented 57% of the digestion products. The results demonstrate that this hyaluronate is an unbranched polymer of approximatey equal amounts of glucuronic acid and N-acetylglucosamine. The data also indicate that if this glycosaminoglycan contains any of the neutral sugars for which it was analysed, their concentration must be less than 0.020% of the sum of the known components. |
