| First Author | Eaton RH | Year | 1967 |
| Journal | Biochem J | Volume | 105 |
| Issue | 3 | Pages | 1307-12 |
| PubMed ID | 16742561 | Mgi Jnum | J:336433 |
| Mgi Id | MGI:7489778 | Doi | 10.1042/bj1051307 |
| Citation | Eaton RH, et al. (1967) Organic pyrophosphates as substrates for human alkaline phosphatases. Biochem J 105(3):1307-12 |
| abstractText | 1. Purified human liver and small-intestinal alkaline orthophosphatases release inorganic phosphate at appreciable rates from a variety of organic pyrophosphate substrates. 2. The pyrophosphatase action is inhibited by Mg(2+) ions at concentrations that activate the hydrolysis of orthophosphate substrates by these enzymes. 3. The results of mixed-substrate experiments, denaturation studies with heat or urea and starch-gel electrophoresis suggest that both orthophosphatase and pyrophosphatase activities are, in each preparation, properties of a single enzyme. 4. Intestinal phosphatase shows greater pyrophosphatase activity relative to orthophosphatase than the liver enzyme. |
