| First Author | Kanemaru A | Year | 2013 |
| Journal | Biosci Biotechnol Biochem | Volume | 77 |
| Issue | 7 | Pages | 1575-8 |
| PubMed ID | 23832333 | Mgi Jnum | J:355660 |
| Mgi Id | MGI:7751514 | Doi | 10.1271/bbb.130070 |
| Citation | Kanemaru A, et al. (2013) High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system. Biosci Biotechnol Biochem 77(7):1575-8 |
| abstractText | Small ubiquitin-related modifier (SUMO) research requires large amounts of SUMO-activating enzyme (SUMO-E1) for in vitro SUMOylation assays. In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His6-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His6-mAU was obtained from 100 mL of Sf9 cell culture. The recombinant protein had SUMO-E1 activity in multiple in vitro SUMOylation assays. |
