| First Author | Uchimura Y | Year | 2004 |
| Journal | FEBS Lett | Volume | 564 |
| Issue | 1-2 | Pages | 85-90 |
| PubMed ID | 15094046 | Mgi Jnum | J:359368 |
| Mgi Id | MGI:7787302 | Doi | 10.1016/S0014-5793(04)00321-7 |
| Citation | Uchimura Y, et al. (2004) Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway. FEBS Lett 564(1-2):85-90 |
| abstractText | Here, we developed a binary vector system that introduces a synthetic SUMO-1 conjugation pathway into Escherichia coli and demonstrated that large amounts of sumoylated Ran GTPase activating protein 1 C-terminal region (RanGAP1-C2), Ran binding protein 2 internal repeat domain, p53 and promyelocytic leukemia were efficiently produced. The sumoylated recombinant RanGAP1-C2 appeared to retain the in vivo properties, since it was specifically sumoylated at lysine 517 as expected from in vivo studies. Our findings indicate the establishment of a biosynthetic route for producing large amounts of sumoylated recombinant proteins that will open up new avenues for studying the biochemical and structural aspects of the SUMO-1 modification pathway. |
