| First Author | Huang CR | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 15 | Pages | 3323-30 |
| PubMed ID | 20627101 | Mgi Jnum | J:163471 |
| Mgi Id | MGI:4822083 | Doi | 10.1016/j.febslet.2010.07.013 |
| Citation | Huang CR, et al. (2010) The fast-mobility isoform of mouse Mcl-1 is a mitochondrial matrix-localized protein with attenuated anti-apoptotic activity. FEBS Lett 584(15):3323-30 |
| abstractText | The full-length pro-survival protein Mcl-1 predominantly resides on the outer membrane of mitochondria. Here, we identified a mitochondrial matrix-localized isoform of Mcl-1 that lacks 33 amino acid residues at the N-terminus which serve both as a mitochondrial targeting and processing signal. Ectopically-expressed Mcl-1 without the N-terminal 33 residues failed to enter the mitochondrial matrix but retained wt-like activities both for interaction with BH3-only proteins and anti-apoptosis. In contrast, the mitochondrial matrix-localized isoform failed to interact with BH3-only proteins and manifested an attenuated anti-apoptotic activity. This study reveals that import of Mcl-1 into the mitochondrial matrix results in the attenuation of Mcl-1's anti-apoptotic function. |
