| First Author | Izumi M | Year | 2000 |
| Journal | Biochim Biophys Acta | Volume | 1492 |
| Issue | 2-3 | Pages | 341-52 |
| PubMed ID | 11004506 | Mgi Jnum | J:63626 |
| Mgi Id | MGI:1861311 | Doi | 10.1016/s0167-4781(00)00117-2 |
| Citation | Izumi M, et al. (2000) Transcription of the catalytic 180-kDa subunit gene of mouse DNA polymerase alpha is controlled by E2F, an ets-related transcription factor, and Sp1. Biochim Biophys Acta 1492(2-3):341-52 |
| abstractText | We have isolated a genomic DNA fragment spanning the 5'-end of the gene encoding the catalytic subunit of mouse DNA polymerase alpha. The nucleotide sequence of the upstream region was G/C-rich and lacked a TATA box. Transient expression assays in cycling NIH 3T3 cells demonstrated that the GC box of 20 bp (at nucleotides -112/-93 with respect to the transcription initiation site) and the palindromic sequence of 14 bp (at nucleotides -71/-58) were essential for basal promoter activity. Electrophoretic mobility shift assays showed that Sp1 binds to the GC box. We also purified a protein capable of binding to the palindrome and identified it as GA-binding protein (GABP), an Ets- and Notch-related transcription factor. Transient expression assays in synchronized NIH 3T3 cells revealed that three variant E2F sites near the transcription initiation site (at nucleotides -23/-16, -1/+7 and +17/+29) had no basal promoter activity by themselves, but were essential for growth-dependent stimulation of the gene expression. These data indicate that E2F, GABP and Sp1 regulate the gene expression of this principal replication enzyme. |
