| First Author | Collick A | Year | 1991 |
| Journal | Nucleic Acids Res | Volume | 19 |
| Issue | 23 | Pages | 6399-404 |
| PubMed ID | 1754375 | Mgi Jnum | J:1885 |
| Mgi Id | MGI:50409 | Doi | 10.1093/nar/19.23.6399 |
| Citation | Collick A, et al. (1991) Minisatellite binding protein Msbp-1 is a sequence-specific single-stranded DNA-binding protein. Nucleic Acids Res 19(23):6399-404 |
| abstractText | Msbp-1 is a minisatellite-specific DNA-binding protein. Using synthetic binding substrates, we now show that Msbp-1 binds not to double-stranded DNA, but exclusively to single-stranded DNA. Binding is specific to the guanine-rich strand of the minisatellite duplex, interactions with the cytosine-rich strand being undetectable by southwestern analysis. Furthermore, the binding site required for successful DNA-protein interactions appears to be two or more minisatellite repeat units. We have also isolated, by whole-genome PCR and cloning, one Msbp-1 binding site from the human genome. Again, the binding strand of this molecule contains a repetitive G-rich structure equivalent to that of a small minisatellite. These observations are discussed with respect to other single-stranded DNA-binding proteins known to play a role in recombination processes. |
