| First Author | Wang L | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 325 |
| Issue | 1 | Pages | 302-7 |
| PubMed ID | 15522233 | Mgi Jnum | J:93510 |
| Mgi Id | MGI:3057220 | Doi | 10.1016/j.bbrc.2004.10.028 |
| Citation | Wang L, et al. (2004) Identification of potential nuclear reprogramming and differentiation factors by a novel selection method for cloning chromatin-binding proteins. Biochem Biophys Res Commun 325(1):302-7 |
| abstractText | Nuclear reprogramming is critical for animal cloning and stem cell creation through nuclear transfer, which requires extensive remodeling of chromosomal architecture involving dramatic changes in chromatin-binding proteins. To understand the mechanism of nuclear reprogramming, it is critical to identify chromatin-binding factors specify the reprogramming process. In this report, we have developed a high-throughput selection method, based on T7 phage display and chromatin immunoprecipitation, to isolate chromatin-binding factors expressed in mouse embryonic stem cells using primary mouse embryonic fibroblast chromatin. Seven chromatin-binding proteins have been isolated by this method. We have also isolated several chromatin-binding proteins involved in hepatocyte differentiation. Our method provides a powerful tool to rapidly and selectively identify chromatin-binding proteins. The method can be used to study epigenetic modification of chromatin during nuclear reprogramming, cell differentiation, and transdifferentiation. |
