| First Author | Baas D | Year | 2012 |
| Journal | J Cell Sci | Volume | 125 |
| Issue | Pt 16 | Pages | 3790-800 |
| PubMed ID | 22553210 | Mgi Jnum | J:197772 |
| Mgi Id | MGI:5494518 | Doi | 10.1242/jcs.101048 |
| Citation | Baas D, et al. (2012) CKIP-1 regulates mammalian and zebrafish myoblast fusion. J Cell Sci 125(Pt 16):3790-800 |
| abstractText | Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle development. Within developing fast-twich myotome, Ckip-1 localises at the periphery of fast precursor cells, closed to the plasma membrane. Unlike wild-type myoblasts that form spatially arrayed multinucleated fast myofibres, Ckip-1-deficient myoblasts show a drastic reduction in fusion capacity. A search for CKIP-1 binding partners identified the ARPC1 subunit of Arp2/3 actin nucleation complex essential for myoblast fusion. We demonstrate that CKIP-1, through binding to plasma membrane phosphoinositides via its PH domain, regulates cell morphology and lamellipodia formation by recruiting the Arp2/3 complex at the plasma membrane. These results establish CKIP-1 as a regulator of cortical actin that recruits the Arp2/3 complex at the plasma membrane essential for muscle precursor elongation and fusion. |
