| First Author | García-Sáinz JA | Year | 1994 |
| Journal | Life Sci | Volume | 54 |
| Issue | 25 | Pages | 1995-2003 |
| PubMed ID | 7911220 | Mgi Jnum | J:18949 |
| Mgi Id | MGI:67164 | Doi | 10.1016/0024-3205(94)90134-1 |
| Citation | Garcia-Sainz JA, et al. (1994) Characterization of the hepatic alpha 1B-adrenoceptors of rats, mice and hamsters. Life Sci 54(25):1995-2003 |
| abstractText | The alpha 1-adrenoceptors present in liver membranes from rats, hamsters and mice were characterized using [3H]prazosin. In the liver membranes from the three species a relatively large number of receptors was observed (500-900 fmol/mg of protein) and the affinities for [3H]prazosin were very similar (0.2-0.3 nM). Membrane preincubation with 10 microM chloroethylclonidine markedly decreased [3H]prazosin binding and higher concentrations essentially abolished specific binding of this radioligand. Binding competition experiments indicated the following orders of potency: a) for agonists: oxymetazoline > epinephrine > or = norepinephrine >> methoxamine and b) for antagonists: prazosin > WB 4101 > or = phentolamine = benoxathian > 5-methyl urapidil. The affinity for (+)niguldipine was also low but there was variation between the three species. Total RNA obtained from the liver of these species hybridized with the alpha 1B-adrenergic cDNA probe. The data suggest that these receptors correspond to the alpha 1B subtype. |
