| First Author | Yin X | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 329 |
| Issue | 3 | Pages | 1010-7 |
| PubMed ID | 15752756 | Mgi Jnum | J:97384 |
| Mgi Id | MGI:3575356 | Doi | 10.1016/j.bbrc.2005.02.075 |
| Citation | Yin X, et al. (2005) Identification of novel CBP interacting proteins in embryonic orofacial tissue. Biochem Biophys Res Commun 329(3):1010-7 |
| abstractText | cAMP response element-binding protein (CREB)-binding protein (CBP) plays an important role as a general co-integrator of multiple signaling pathways and interacts with a large number of transcription factors and co-factors, through its numerous protein-binding domains. To identify nuclear factors associated with CBP in developing orofacial tissue, a yeast two-hybrid screen of a cDNA library derived from orofacial tissue from gestational day 11 to 13 mouse embryos was conducted. Using the carboxy terminus (amino acid residues 1676-2441) of CBP as bait, several novel proteins that bind CBP were identified, including an Msx-interacting-zinc finger protein, CDC42 interaction protein 4/thyroid hormone receptor interactor 10, SH3-domain GRB2-like 1, CCR4-NOT transcription complex subunit 3, adaptor protein complex AP-1 beta1 subunit, eukaryotic translation initiation factor 2B subunit 1 (alpha), and cyclin G-associated kinase. Results of the yeast two-hybrid screen were confirmed by glutathione S-transferase pull-down assays. The identification of these proteins as novel CBP-binding partners allows exploration of new mechanisms by which CBP regulates and integrates diverse cell signaling pathways. |
