| First Author | Yang Q | Year | 2000 |
| Journal | Am J Physiol Renal Physiol | Volume | 279 |
| Issue | 4 | Pages | F765-77 |
| PubMed ID | 10997927 | Mgi Jnum | J:65175 |
| Mgi Id | MGI:1913165 | Doi | 10.1152/ajprenal.2000.279.4.F765 |
| Citation | Yang Q, et al. (2000) Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis. Am J Physiol Renal Physiol 279(4):F765-77 |
| abstractText | Expression and role of sodium glucose cotransporter (SGLT-1) in tubulogenesis were investigated during renal development. A mouse SGLT-1 cDNA was cloned, and it had substantial homology with human and rat forms. Four mRNA transcripts were detected, which differed in size from other species. SGLT-1 transcripts were detected at day 13 of gestation, and their expression increased during later stages extending into the postnatal period. A high mRNA and protein expression of SGLT-1 was seen in tubular segments of the inner cortex and outer medulla at day 16, and it was developmentally regulated. Treatment with SGLT-1 antisense selectively decreased the population of tubules in the metanephric explants. Expression of glomerular mRNA and WGA binding were unchanged. SGLT-1 activity, as measured by [(14)C]methyl-alpha-D-glucopyranoside uptake, increased during gestation in the tubular segments where it is expressed. Glucose uptake was inhibited by the treatment with SGLT-1 antisense and D-galactose. The data suggest that SGLT-1 exhibits a restricted spatiotemporal expression with functional activity confined to the corresponding tubular segments, and it selectively maintains renal tubulogenesis during development. |
